Review

ppp 3d matrix (3-D Matrix)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

3-D Matrix ppp 3d matrix
Average cell number, doubling time and cell doubling number ±SD obtained at different time points (48, 72, 144, 216 h) from ADMSCs cultured on 2D plastic surface or <t> 3D </t> fibrin-based gel prepared from <t> PPP </t> (n = 5). Cells were seeded at a density of 6000 cells/cm 2 in 3.5 cm Petri dishes.

Average cell number, doubling time and cell doubling number ±SD obtained at different time points (48, 72, 144, 216 h) from ADMSCs cultured on 2D plastic surface or <t> 3D </t> fibrin-based gel prepared from <t> PPP </t> (n = 5). Cells were seeded at a density of 6000 cells/cm 2 in 3.5 cm Petri dishes.

Ppp 3d Matrix, supplied by 3-D Matrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppp+3d+matrix/pmc07759956-20-0-1?v=3-D+Matrix
Average 90 stars, based on 1 article reviews

ppp 3d matrix - by Bioz Stars, 2026-08

90/100 stars

Images

1) Product Images from "Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures"

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

Journal: Cells

doi: 10.3390/cells9122578

Figure Legend Snippet: Average cell number, doubling time and cell doubling number ±SD obtained at different time points (48, 72, 144, 216 h) from ADMSCs cultured on 2D plastic surface or 3D fibrin-based gel prepared from PPP (n = 5). Cells were seeded at a density of 6000 cells/cm 2 in 3.5 cm Petri dishes.

Techniques Used: Cell Culture

Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) obtained from cells maintained on plastic surface or inside a 3D matrix prepared from PPP, at different time points (48, 72, 144, 216 h) (n = 5). Statistically significant differences are indicated as follows: ** p ≤ 0.01, **** p ≤ 0.0001.

Figure Legend Snippet: Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) obtained from cells maintained on plastic surface or inside a 3D matrix prepared from PPP, at different time points (48, 72, 144, 216 h) (n = 5). Statistically significant differences are indicated as follows: ** p ≤ 0.01, **** p ≤ 0.0001.

Techniques Used:

Morphology of ADMSCs grown inside a 3D matrix prepared from platelet-rich plasma (PRP) ( a1 , a2 , a3 ; 100×) or PPP ( b1 , b2 , b3 ; 100×), at different time points (0, 72, 144 h). The initial round-shaped morphology (a1, b1; time = 0 h), is changed in a spindle, fibroblast-like morphology at 72 and 144 h ( a2 , b2 , a3 , and b3 ). Cells gradually populate the whole 3D fibrin-derived scaffold, spreading over the entire thickness. No morphological differences are observed between cells maintained in PRP or platelet-poor plasma (PPP) derived matrix.

Figure Legend Snippet: Morphology of ADMSCs grown inside a 3D matrix prepared from platelet-rich plasma (PRP) ( a1 , a2 , a3 ; 100×) or PPP ( b1 , b2 , b3 ; 100×), at different time points (0, 72, 144 h). The initial round-shaped morphology (a1, b1; time = 0 h), is changed in a spindle, fibroblast-like morphology at 72 and 144 h ( a2 , b2 , a3 , and b3 ). Cells gradually populate the whole 3D fibrin-derived scaffold, spreading over the entire thickness. No morphological differences are observed between cells maintained in PRP or platelet-poor plasma (PPP) derived matrix.

Techniques Used: Clinical Proteomics, Derivative Assay

Average cell number, doubling time and cell doubling number obtained at different time points (72, 144 h) from ADMSCs cultured inside a 3D fibrin-based matrix prepared from PRP or PPP (n = 3).

Figure Legend Snippet: Average cell number, doubling time and cell doubling number obtained at different time points (72, 144 h) from ADMSCs cultured inside a 3D fibrin-based matrix prepared from PRP or PPP (n = 3).

Techniques Used: Cell Culture

Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) of cells maintained inside a 3D matrix prepared from PRP or PPP, at different time points (72, 144 h) (n = 3). No statistically significant differences are observed between the two culture conditions.

Figure Legend Snippet: Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) of cells maintained inside a 3D matrix prepared from PRP or PPP, at different time points (72, 144 h) (n = 3). No statistically significant differences are observed between the two culture conditions.

Techniques Used:

$Morphology of adipose tissue derived stromal vascular fraction (SVF) cells (100×), expanded on the plastic surface ( a ) or within a 3D matrix prepared from PPP. ( b ) The cells were expanded until they reached about 90% of confluence on plastic (average 192 h). The morphology resembles what observed for ADMSCs cultures in the two different environments.$
Figure Legend Snippet: Morphology of adipose tissue derived stromal vascular fraction (SVF) cells (100×), expanded on the plastic surface ( a ) or within a 3D matrix prepared from PPP. ( b ) The cells were expanded until they reached about 90% of confluence on plastic (average 192 h). The morphology resembles what observed for ADMSCs cultures in the two different environments.

Techniques Used: Derivative Assay

The average number of cells and cell number fold increase obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP. The cells were counted when plastic cultures reached about 90% of confluency (average 192 h) (n = 3).

Figure Legend Snippet: The average number of cells and cell number fold increase obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP. The cells were counted when plastic cultures reached about 90% of confluency (average 192 h) (n = 3).

Techniques Used: Derivative Assay

Graph bars representing the average number of cells obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP ( n = 3). Statistically significant differences are indicated as follows: ** p ≤ 0.01.

Figure Legend Snippet: Graph bars representing the average number of cells obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP ( n = 3). Statistically significant differences are indicated as follows: ** p ≤ 0.01.

Techniques Used: Derivative Assay

Evaluation of ADMSCs growth on plastic surface or inside the 3D fibrin-based matrix prepared from PPP, by MTT assay at 48 ( a ), 72 ( b ), and 96 ( c ) hours. The number of cells seeded was 5000/well (blue bars) or 10,000/well (yellow bars). The metabolic MTT assay confirmed the results observed by direct cell count. 3D fibrin-based matrix is a suitable environment to stimulate ADMSCs’ growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001.

Figure Legend Snippet: Evaluation of ADMSCs growth on plastic surface or inside the 3D fibrin-based matrix prepared from PPP, by MTT assay at 48 ( a ), 72 ( b ), and 96 ( c ) hours. The number of cells seeded was 5000/well (blue bars) or 10,000/well (yellow bars). The metabolic MTT assay confirmed the results observed by direct cell count. 3D fibrin-based matrix is a suitable environment to stimulate ADMSCs’ growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001.

Techniques Used: MTT Assay, Cell Counting

( a ) Comparison between FBS and allogeneic canine serum as a medium supplement for ADMSCs culture, by MTT assay (5000 cells/well; 72 h). When seeded on plastic (blue bars), supplementation of medium with 10% allogeneic serum induced a statistically significant stimulus to cell growth with respect to FBS. When cells were cultured inside a 3D fibrin-based matrix prepared from PPP (yellow bars), allogeneic serum, and FBS supplementation, or no supplementation in the culture medium, did not induce different cell growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 (n = 3). ( b ) Autologous serum as a substitute for FBS in medium supplementation for ADMSCs growth (5000 cells/well; 72 h). When cells were cultivated on plastic surface (blue bars), 10% autologous serum medium supplementation was an effective substitute for 10% FBS supplementation. When cells were seeded inside a 3D fibrin-based matrix (yellow bars), no serum supplementation, FBS or autologous serum supplementation induced comparable results. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 ( n = 3).

Figure Legend Snippet: ( a ) Comparison between FBS and allogeneic canine serum as a medium supplement for ADMSCs culture, by MTT assay (5000 cells/well; 72 h). When seeded on plastic (blue bars), supplementation of medium with 10% allogeneic serum induced a statistically significant stimulus to cell growth with respect to FBS. When cells were cultured inside a 3D fibrin-based matrix prepared from PPP (yellow bars), allogeneic serum, and FBS supplementation, or no supplementation in the culture medium, did not induce different cell growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 (n = 3). ( b ) Autologous serum as a substitute for FBS in medium supplementation for ADMSCs growth (5000 cells/well; 72 h). When cells were cultivated on plastic surface (blue bars), 10% autologous serum medium supplementation was an effective substitute for 10% FBS supplementation. When cells were seeded inside a 3D fibrin-based matrix (yellow bars), no serum supplementation, FBS or autologous serum supplementation induced comparable results. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 ( n = 3).

Techniques Used: Comparison, MTT Assay, Cell Culture

Image Search Results

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Average cell number, doubling time and cell doubling number ±SD obtained at different time points (48, 72, 144, 216 h) from ADMSCs cultured on 2D plastic surface or 3D fibrin-based gel prepared from PPP (n = 5). Cells were seeded at a density of 6000 cells/cm 2 in 3.5 cm Petri dishes.

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: Cell Culture

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) obtained from cells maintained on plastic surface or inside a 3D matrix prepared from PPP, at different time points (48, 72, 144, 216 h) (n = 5). Statistically significant differences are indicated as follows: ** p ≤ 0.01, **** p ≤ 0.0001.

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques:

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Morphology of ADMSCs grown inside a 3D matrix prepared from platelet-rich plasma (PRP) ( a1 , a2 , a3 ; 100×) or PPP ( b1 , b2 , b3 ; 100×), at different time points (0, 72, 144 h). The initial round-shaped morphology (a1, b1; time = 0 h), is changed in a spindle, fibroblast-like morphology at 72 and 144 h ( a2 , b2 , a3 , and b3 ). Cells gradually populate the whole 3D fibrin-derived scaffold, spreading over the entire thickness. No morphological differences are observed between cells maintained in PRP or platelet-poor plasma (PPP) derived matrix.

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: Clinical Proteomics, Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Average cell number, doubling time and cell doubling number obtained at different time points (72, 144 h) from ADMSCs cultured inside a 3D fibrin-based matrix prepared from PRP or PPP (n = 3).

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: Cell Culture

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) of cells maintained inside a 3D matrix prepared from PRP or PPP, at different time points (72, 144 h) (n = 3). No statistically significant differences are observed between the two culture conditions.

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques:

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Morphology of adipose tissue derived stromal vascular fraction (SVF) cells (100×), expanded on the plastic surface ( a ) or within a 3D matrix prepared from PPP. ( b ) The cells were expanded until they reached about 90% of confluence on plastic (average 192 h). The morphology resembles what observed for ADMSCs cultures in the two different environments.

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: The average number of cells and cell number fold increase obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP. The cells were counted when plastic cultures reached about 90% of confluency (average 192 h) (n = 3).

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Graph bars representing the average number of cells obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP ( n = 3). Statistically significant differences are indicated as follows: ** p ≤ 0.01.

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Evaluation of ADMSCs growth on plastic surface or inside the 3D fibrin-based matrix prepared from PPP, by MTT assay at 48 ( a ), 72 ( b ), and 96 ( c ) hours. The number of cells seeded was 5000/well (blue bars) or 10,000/well (yellow bars). The metabolic MTT assay confirmed the results observed by direct cell count. 3D fibrin-based matrix is a suitable environment to stimulate ADMSCs’ growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001.

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: MTT Assay, Cell Counting

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: ( a ) Comparison between FBS and allogeneic canine serum as a medium supplement for ADMSCs culture, by MTT assay (5000 cells/well; 72 h). When seeded on plastic (blue bars), supplementation of medium with 10% allogeneic serum induced a statistically significant stimulus to cell growth with respect to FBS. When cells were cultured inside a 3D fibrin-based matrix prepared from PPP (yellow bars), allogeneic serum, and FBS supplementation, or no supplementation in the culture medium, did not induce different cell growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 (n = 3). ( b ) Autologous serum as a substitute for FBS in medium supplementation for ADMSCs growth (5000 cells/well; 72 h). When cells were cultivated on plastic surface (blue bars), 10% autologous serum medium supplementation was an effective substitute for 10% FBS supplementation. When cells were seeded inside a 3D fibrin-based matrix (yellow bars), no serum supplementation, FBS or autologous serum supplementation induced comparable results. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 ( n = 3).

Article Snippet: PPP 3D matrix , 406,889 ± 141,420 , 2,623,334 ± 1,470,181.

Techniques: Comparison, MTT Assay, Cell Culture

Review

Structured Review

Images

1) Product Images from "Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures"

Similar Products

Image Search Results